online tool for restriction analysis of two sequences
compare restriction profiles
restriction digest with gel electrophoresis simulation
compare restriction patterns online utility
restriction enzymes patterns dna fragment size calculation
web app to compare restriction sites
restriction profiles comparison online tool
restriction mapping of plasmid dna
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DNA SEQUENCE INPUT
COMMONLY USED ENZYMES
This is a manual selection of the most frequently used enzymes, typically those whose recognition sites are present in the multiple cloning sites region of commonly used plasmid vectors plus some other enzymes traditionally used for restriction analysis and molecular cloning.
The Restriction Comparator application contains a list of all commercially available restriction enzymes. From these 543 enzymes, you can select a subset based on the length of recognition sites or a presence of ambiguous positions in them.
If a specific set of restriction enzymes is to be used in the analyses, one can either enter the list (paste it in the text area in the "Enter the restriction enzyme list" dialog) or load a list of enzymes provided as a plain text file with one restriction enzyme name per line, for example:
DNA SOURCE GENOTYPE
Plasmid DNA isolated from E. coli is methylated on Dam and Dcm sites unless a dam-/dcm- strain has been used. DNA obtained with PCR amplification is rid of all methylation so "dam- " and "dcm- " should be checked in such cases.
TARGET FRAGMENT RANGE
For a cleavage pattern to be suggested as discerning, at least one fragment within the target range must be produced by such cleavage in at least one sequence.
MINIMUM FRAGMENT SIZE DIFFERENCE
For a cleavage pattern to be suggested as discerning, at least one fragment within the target range must differ from each fragment from the other sequence by at least the entered number of percents of the size of that fragment.