Restriction Analyzer will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence.
It produces a variety of outputs including restriction enzyme maps and theoretical digests presented as restriction fragment list and a simulated electropherogram. Thus the output of the program is an online restriction profile of a DNA sequence.
Restriction digest is a method that uses several restriction enzymes to detect variations from the expected digestion patterns of DNA sequences. Restriction Analyzer is a restriction profiles online program and restriction digest simulator.
Restriction Analyzer provides an online restriction site analysis and can also be seen as a restriction fragment calculator. As a result, the user will get restriction enzymes patterns and DNA fragment size calculation.
Restriction digests are mixtures of DNA fragments produced by the reaction of DNA and a restriction enzyme, an enzyme that cuts at specific base sequences. These enzymes are used for the creation of genetic maps prior to sequencing.
To perform restriction mapping of plasmid DNA and find restriction enzyme sites, use Restriction Analyzer.
Restriction Analyzer can also display restriction enzymes sequence and perform restrictase screen and restriction digestion.
In summary, Restriction Analyzer is a valuable molecular biology software, a web app, also molecular cloning software tool which enables the user to find restriction sites (restriction endonuclease recognition patterns) and is an important modern alternative to older programs like NEBcutter, RestrictionMapper, or WebCutter. In other words, Restriction Analyzer is a restriction patterns online utility.
Restriction Analyzer can use a user-provided list of restriction enzymes.
DNA source genotype:
COMMONLY USED ENZYMES
This is a manual selection of the most frequently used enzymes, typically those whose recognition sites are present in the multiple cloning sites region of commonly used plasmid vectors plus some other enzymes traditionally used for restriction analysis and molecular cloning.
The Restriction Analyzer application contains a list of all commercially available restriction enzymes. From these 549 enzymes, you can select a subset based on the length of recognition sites or a presence of ambiguous positions in them.
If a specific set of restriction enzymes is to be used in the analyses, one can either enter the list (paste it in the text area in the "Enter the restriction enzyme list" dialog) or load a list of enzymes provided as a plain text file with one restriction enzyme name per line, for example:
DNA SOURCE GENOTYPE
Plasmid DNA isolated from E. coli is methylated on Dam and Dcm sites unless a dam-/dcm- strain has been used. DNA obtained with PCR amplification is rid of all methylation so "dam- " and "dcm- " should be checked in such cases.
|B||C, G, T||V|
|D||A, G, T||H|
|H||A, C, T||D|
|V||A, C, G||B|
|N||A, C, G, T||N|