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DNA Calculator

Open a sequence file (txt, fasta or GenBank format) or paste a sequence in the area bellow.
Name:
DNA RNA

single strand double strand

linear circular

5' hydroxyl 5' phosphate 5' triphosphate

Length:

GC content:

Molecular weight:

Molarity of 1 μg/μl solution:

Number of molecules in 1 μg:

Approx. 260 nm extinction coefficient:

A260 of 1 μg/μl solution after 100-fold dilution:

In the following lines, fill in two out of the three fields and click 'Calculate'.

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Absorbance and concentration

In a 1 cm cuvette, 260 nm absorbance after dilution by factor corresponds to original concentration of μg/μl.


For the calculation use:
empirical rules predicted extinction coefficient ?

Empirical rules for the relationship between nucleic acid concentration and 260 nm absorbance (OD260)

260 nm absorbance value 1 (alias "1 OD260 unit") corresponds to following concentrations:
  • 50 μg/ml dsDNA
  • 33 μg/ml ssDNA
  • 20 μg/ml ssDNA oligo
  • 40 μg/ml RNA

OD260 units are sometimes used to describe quantities of nucleic acids. One OD260 unit is the amount of nucleic acid that will give 260 nm absorbance 1 after dissolution in 1 ml of solvent.

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Molarity, mass and volume

For micromolar concentration dissolve μg in the volume of μl.

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Base-pair molarity of dsDNA solutions

For millimolar concentration of base pairs dissolve μg of dsDNA in the volume of μl.

Online DNA calculator

Online DNA calculations

DNA MW calculation

DNA molecular weight calculation

DNA molarity calculation

DNA base pair molarity calculation

DNA concentration calculation

DNA MW calculator

Calculate DNA MW

Calculate DNA molecular weight

DNA molarity calculator

DNA base pair molarity calculator

DNA concentration calculator

DNA GC content calculator

Number of DNA molecules calculation

OD260 calculator


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