Henderson-Hasselbalch equation calculator

Henderson-Hasselbalch equation allows to calculate a buffer pH value.

online buffer calculator

online pH calculator

calculate pH of a buffer

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Buffer Calculator

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Henderson-Hasselbalch Equation

Results obtained from Henderson-Hasselbalch equation are approximate and only valid for diluted solutions (< 100mM) and in the pH range of pKa ± 1. pH value is a reverse function of temperature (pH decreases with increasing temperature and vice versa). pH value may change after dilution of concentrated buffer stocks. pH value may be affected by salts and other solutes. It is recommended to check the actual pH value with a pH meter.

Fill all but one field and click 'Calculate':

pH = pKa + log
[A-]
[HA]
[A-] - concentration of the conjugate base
[HA] - concentration of the conjugate acid

pKa values of common buffers

BufferUseful pH RangepKa (at 25°C)
Phosphate1.7 - 2.9, 5.8 - 8.02.15, 7.20, 12.33
Glycine2.2 - 3.6, 8.8 - 10.62.35, 9.78
Citrate3.0 - 6.23.13, 4.76, 6.40
Acetate3.7 - 5.64.76
MES5.5 - 6.76.10
Bis-Tris5.8 - 7.26.50
Carbonate6.0 - 8.0, 9.5 - 11.16.35, 10.33
ADA6.0 - 7.26.59
ACES6.1 - 7.56.78
PIPES6.1 - 7.56.76
MOPSO6.2 - 7.66.90
Imidazole6.2 - 7.86.95
Bis-Tris Propane6.3 - 9.56.8, 9.0
BES6.4 - 7.87.09
MOPS6.5 - 7.97.20
TES6.8 - 8.27.40
HEPES6.8 - 8.27.48
DIPSO7.0 - 8.27.60
MOBS6.9 - 8.37.60
TAPSO7.0 - 8.27.60
Tris7.0 - 9.08.06
HEPPSO7.1 - 8.57.80
POPSO7.2 - 8.57.80
TEA7.3 - 8.37.80
EPPS7.3 - 8.78.00
Tricine7.4 - 8.88.05
Gly-Gly7.5 - 8.98.20
Bicine7.6 - 9.08.26
HEPBS7.6 - 9.08.30
TAPS7.7 - 9.18.40
AMPD7.8 - 9.78.80
TABS8.2 - 9.68.90
AMPSO8.3 - 9.79.00
Boric acid8.5 - 10.29.23, 12.74, 13.80
CHES8.6 - 10.09.49
CAPSO8.9 - 10.39.60
AMP9.0 - 10.59.70
CAPS9.7 - 11.110.40
CABS10.0 - 11.410.70

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DISCLAIMER:This free software comes without any warranty. The author of the software bears no responsibility for any loss or damage that may arise from its use for any purpose.