buffer,pH,Henderson-Hasselbalch equation calculator

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Buffer Calculator

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Henderson-Hasselbalch Equation

Results obtained from Henderson-Hasselbalch equation are approximate and only valid for diluted solutions (< 100mM) and in the pH range of pKa ± 1. pH value is a reverse function of temperature (pH decreases with increasing temperature and vice versa). pH value may change after dilution of concentrated buffer stocks. pH value may be affected by salts and other solutes. It is recommended to check the actual pH value with a pH meter.

Fill all but one field and click 'Calculate':

pH = pKa + log
cA
cHA
cA - concentration of the conjugated base
cHA - concentration of the conjugated acid

pKa values of common buffers

BufferUseful pH RangepKa (at 25°C)
Phosphate1.7 - 2.9, 5.8 - 8.02.15, 7.20, 12.33
Glycine2.2 - 3.6, 8.8 - 10.62.35, 9.78
Citrate3.0 - 6.23.13, 4.76, 6.40
Acetate3.7 - 5.64.76
MES5.5 - 6.76.10
Bis-Tris5.8 - 7.26.50
Carbonate6.0 - 8.0, 9.5 - 11.16.35, 10.33
ADA6.0 - 7.26.59
ACES6.1 - 7.56.78
PIPES6.1 - 7.56.76
MOPSO6.2 - 7.66.90
Imidazole6.2 - 7.86.95
Bis-Tris Propane6.3 - 9.56.8, 9.0
BES6.4 - 7.87.09
MOPS6.5 - 7.97.20
TES6.8 - 8.27.40
HEPES6.8 - 8.27.48
DIPSO7.0 - 8.27.60
MOBS6.9 - 8.37.60
TAPSO7.0 - 8.27.60
Tris7.0 - 9.08.06
HEPPSO7.1 - 8.57.80
POPSO7.2 - 8.57.80
TEA7.3 - 8.37.80
EPPS7.3 - 8.78.00
Tricine7.4 - 8.88.05
Gly-Gly7.5 - 8.98.20
Bicine7.6 - 9.08.26
HEPBS7.6 - 9.08.30
TAPS7.7 - 9.18.40
AMPD7.8 - 9.78.80
TABS8.2 - 9.68.90
AMPSO8.3 - 9.79.00
Boric acid8.5 - 10.29.23, 12.74, 13.80
CHES8.6 - 10.09.49
CAPSO8.9 - 10.39.60
AMP9.0 - 10.59.70
CAPS9.7 - 11.110.40
CABS10.0 - 11.410.70

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